abstract：:Fluorescent and incandescent lighting systems were applied for batch photofermentative hydrogen production by four purple non-sulfur photosynthetic bacteria (PNSB). The hydrogen production efficiency of Rhodopseudomonas palustris, Rhodobacter sphaeroides, Rhodobacter capsulatus, and Rhodospirillum rubrum was evaluated...

journal_title：Applied biochemistry and biotechnology

authors： Hu C,Choy SY,Giannis A

更新日期：2018-05-01 00:00:00

abstract：:Ovarian malignancy is diagnosed in nearly a fourth of a million women internationally every year. Methylation of RASSF1A tumor suppressor gene prompts its inactivation in diseases. In this study, the RASSF1A promoter methylation was detected by methylated-specific PCR and investigated serum RASSF1A protein level throu...

journal_title：Applied biochemistry and biotechnology

authors： Rezk NA,Mohamed RH,Alnemr AA,Harira M

更新日期：2018-05-01 00:00:00

abstract：:Lipase from Rhizomucor miehei (RML) was immobilized onto chitosan support in the presence of some surfactants added at low levels using two different strategies. In the first approach, the enzyme was immobilized in the presence of surfactants on chitosan supports previously functionalized with glutaraldehyde. In the s...

journal_title：Applied biochemistry and biotechnology

authors： de Oliveira UMF,Lima de Matos LJB,de Souza MCM,Pinheiro BB,Dos Santos JCS,Gonçalves LRB

更新日期：2018-04-01 00:00:00

abstract：:(R)-3, 5-Bis(trifluoromethyl)phenyl ethanol is a key chiral intermediate for the synthesis of aprepitant. Through a genome mining approach, an NADPH-dependent short-chain dehydrogenases derived from Burkholderia cenocepacia (Bc-SDR) was discovered with excellent anti-Prelog's stereoselectivity of reducing 3, 5-bis(tri...

journal_title：Applied biochemistry and biotechnology

authors： Yu S,Li H,Lu Y,Zheng G

更新日期：2018-04-01 00:00:00

abstract：:Prostate apoptosis response-4 (Par-4), an anticancer protein that interacts with cell surface receptor GRP78, can selectively suppress proliferation and induce apoptosis of cancer cells. The core domain of Par-4 (aa 137-195), designated as SAC, is sufficient to inhibit tumor growth and metastasis without harming norma...

journal_title：Applied biochemistry and biotechnology

authors： Zhang J,Sun A,Dong Y,Wei D

更新日期：2018-04-01 00:00:00

abstract：:Neuraminidase (NA), a surface protein, facilitates the release of nascent virus and thus spreads infection. It has been renowned as a potential drug target for influenza A virus infection. The drugs such as oseltamivir, zanamivir, peramivir, and laninamivir are approved for the treatment of influenza infection. Additi...

journal_title：Applied biochemistry and biotechnology

authors： K R,V S

更新日期：2018-04-01 00:00:00

abstract：:Fed-batch method has shown a great promise in debottlenecking the high-solid enzymatic hydrolysis for the commercialization of cellulosic biosugar conversion for biofuel/biochemical production. To further improve enzymatic hydrolysis efficiency at high solid loading, fed-batch methods of green liquor-pretreated hardwo...

journal_title：Applied biochemistry and biotechnology

authors： He L,Han Q,Jameel H,Chang HM,Phillips R,Wang Z

更新日期：2018-04-01 00:00:00

abstract：:The amylase from Aureobasidium pullulans Cau 19 was purified by ammonium sulfate precipitation and Sephadex G-100 chromatography with a 9.25-fold increase in specific activity as compared to crude enzyme. Km and turn over values of the enzyme were 6.25 mg/mL and 5.0 × 102/min, respectively. Effect of different metal i...

journal_title：Applied biochemistry and biotechnology

authors： Mulay YR,Deopurkar RL

更新日期：2018-02-01 00:00:00

abstract：:Bacterial strain NYT501, which we previously isolated from soil, was identified as Stenotrophomonas maltophilia, and it was confirmed that this strain produces an intracellular β-N-acetylhexosaminidase exhibiting transglycosylation activity. Several properties of this enzyme were characterized using a partially purifi...

journal_title：Applied biochemistry and biotechnology

authors： Uehara A,Takahashi N,Moriyama M,Hirano T,Hakamata W,Nishio T

更新日期：2018-02-01 00:00:00

abstract：:Aldehyde dehydrogenase (E.C. 1.2.1.x) can catalyze detoxification of acetaldehydes. A novel acetaldehyde dehydrogenase (istALDH) from the non-Saccharomyces yeast Issatchenkia terricola strain XJ-2 has been previously characterized. In this work, Lactococcus lactis with the NIsin Controlled Expression (NICE) System was...

journal_title：Applied biochemistry and biotechnology

authors： Lyu Y,LaPointe G,Zhong L,Lu J,Zhang C,Lu Z

更新日期：2018-02-01 00:00:00

abstract：:Eighty-nine cultured Pseudomonas species isolated from the sediment and water samples collected from five industrial Red Sea regions that have been affected by petroleum and industry. Genotypic (exoT, exoS, exoU, exoY, lasA, lasB, rhlA, rhlB, Pf1, PAGI-1, -2, and -3) and phenotypic (DNase, elastase, lipase, protease, ...

journal_title：Applied biochemistry and biotechnology

authors： El-Gendy MMAA,Al-Zahrani HAA,Abozinadah NY,El-Bondkly AMA

更新日期：2018-01-01 00:00:00

abstract：:The merit of deacetylation of corn stover prior to pretreatment is decreasing the formation of inhibitors and improving enzyme hydrolysis, proved in dilute acid pretreatment. However, few studies are done on how deacetylation would affect bioconversion process containing steam explosion. In this study, the effect of d...

journal_title：Applied biochemistry and biotechnology

authors： Tang Y,Dou X,Hu J,Jiang J,Saddler JN

更新日期：2018-01-01 00:00:00

abstract：:γ-Aminobutyric acid (γ-GABA) is a non-proteinogenic amino acid, which acts as a major regulator in the central nervous system. Glutamate decarboxylase (namely GAD, EC 4.1.1.15) is known to be an ideal enzyme for γ-GABA production using L-glutamic acid as substrate. In this study, we cloned and expressed GAD gene from ...

journal_title：Applied biochemistry and biotechnology

authors： Xiong Q,Xu Z,Xu L,Yao Z,Li S,Xu H

更新日期：2017-12-01 00:00:00

abstract：:The glpX gene from Francisella tularensis encodes for the class II fructose 1,6-bisphosphatase (FBPaseII) enzyme. The glpX gene has been verified to be essential in F. tularensis, and the inactivation of this gene leads to impaired bacterial growth on gluconeogenic substrates. In the present work, we have complemented...

journal_title：Applied biochemistry and biotechnology

authors： Gutka HJ,Wolf NM,Bondoc JMG,Movahedzadeh F

更新日期：2017-12-01 00:00:00

abstract：:Performance of microbial carbon capture cells (MCCs), having a low-cost clayware separator, was evaluated in terms of wastewater treatment and electricity generation using algae Chlorella pyrenoidosa in MCC-1 and Anabaena ambigua in MCC-2 and without algae in a cathodic chamber of MCC-3. Higher power production was ac...

journal_title：Applied biochemistry and biotechnology

authors： Jadhav DA,Jain SC,Ghangrekar MM

更新日期：2017-11-01 00:00:00

abstract：:Molecular chaperones and protein folding factors of bacterial periplasmic space play important roles in assisting disulfide bond formation and proper protein folding. In this study, effects of disulfide bond protein (Dsb) families were investigated on assembly of 3F3 Fab, an antibody inhibitor targeting matrix metallo...

journal_title：Applied biochemistry and biotechnology

authors： Rodriguez C,Nam DH,Kruchowy E,Ge X

更新日期：2017-10-01 00:00:00

abstract：:In the present work, protective effect of tetrahydrocurcumin (THC) against oxidative damages in cadmium (Cd)-induced toxicity in rats was evaluated. Cd is an important environmental and industrial toxicant that affects almost all the organs, especially liver. Liver is the major organ responsible for the metabolism and...

journal_title：Applied biochemistry and biotechnology

authors： Ramakrishnan R,Elangovan P,Pari L

更新日期：2017-09-01 00:00:00

abstract：:During the aging of yeast culture, Kluyveromyces marxianus undergoes a number of changes in physiology and these changes play a significant role during fermentation. Aged stationary phase cells were found to contain more reactive oxygen species. Additionally, the level of oxidant is counteracted by the antioxidant def...

journal_title：Applied biochemistry and biotechnology

authors： Saini P,Beniwal A,Vij S

更新日期：2017-09-01 00:00:00

abstract：:Sweet sorghum (Sorghum sp.) has high biomass yield. Hydrolysis of lignocellulosic sweet sorghum bagasse (SSB) to fermentable sugar could be useful for manufacture of biofuel or other fermentation products. Pretreatment of lignocellulosic biomass to degrade lignin before enzymatic hydrolysis is a key step. Fungal pretr...

journal_title：Applied biochemistry and biotechnology

authors： Mishra V,Jana AK

更新日期：2017-09-01 00:00:00

abstract：:Unlike silkworm (Bombyx mori) fibroin (SF), weaver ant (Oecophylla smaragdina) fibroin (WAF) is much less studied. Due to differences in amino acid composition and protein structure, this work aimed to produce the recombinant WAF protein, designated as WAF1, and investigated on its potential application as a biomateri...

journal_title：Applied biochemistry and biotechnology

authors： Khamhaengpol A,Siri S

更新日期：2017-09-01 00:00:00

abstract：:Bacteria isolated from thermophilic environment that can produce cellulase as well as utilise agro-waste biomass have a high potential for developing thermostable cellulase required in the biofuel industry. The cost for cellulase represents a significant challenge in converting lignocellulose to fermentable sugars for...

journal_title：Applied biochemistry and biotechnology

authors： Kazeem MO,Shah UKM,Baharuddin AS,AbdulRahman NA

更新日期：2017-08-01 00:00:00

abstract：:The enzyme 3-hydroxy-3-methyl-glutaryl CoA reductase (HMGR) is a glycoprotein of the endoplasmic reticulum that participates in the mevalonate pathway, the precursor of cholesterol in human and ergosterol in fungi. This enzyme has three domains: transmembrane, binding, and soluble. In this study, we expressed and puri...

journal_title：Applied biochemistry and biotechnology

authors： Andrade-Pavón D,Cuevas-Hernández RI,Trujillo-Ferrara JG,Hernández-Rodríguez C,Ibarra JA,Villa-Tanaca L

更新日期：2017-08-01 00:00:00

abstract：:Fluorescence spectroscopy, UV-visible absorption spectroscopy, circular dichroism (CD) spectroscopy, viscometry, cyclic voltammetry (CV), and differential pulse voltammetry (DPV) were applied to investigate the competitive interaction of DNA with two aromatic α-aminobisphosphonates and neutral red dye (NR, intercalato...

journal_title：Applied biochemistry and biotechnology

authors： Gholivand MB,Peyman H,Gholivand K,Roshanfekr H,Taherpour AA,Yaghobi R

更新日期：2017-07-01 00:00:00

abstract：:The nuclear receptor Rev-erbβ is a potent transcriptional factor whose functional study has been limited by the lack of suitable antibodies against it. To better understand Rev-erbβ's biological roles, we generated five hybridoma cell lines secreting antibodies against human Rev-erbβ in mice immunized with the purifie...

journal_title：Applied biochemistry and biotechnology

authors： Chen F,Li Y,Zhao J,Mao Q,Xia H

更新日期：2017-07-01 00:00:00

abstract：:MicroRNAs are small non-coding RNAs which play significant role in RNA interference. The present work deals with mining of the conserved miRNA and their target genes from the contigs, ESTs, and BAC end sequences of commercially important catfish, Clarias batrachus, from India. A total of 138, 1 and 1 conserved pre-miR...

journal_title：Applied biochemistry and biotechnology

authors： Agarwal S,Nagpure NS,Srivastava P,Kumar R,Pandey M,Srivastava S,Jena JK,Das P,Kushwaha B

更新日期：2017-07-01 00:00:00

abstract：:The stabilization of olive recombinant hydroperoxide lyases (rHPLs) was investigated using selected chemical additives. Two rHPLs were studied: HPL full-length and HPL with its chloroplast transit peptide deleted (matured HPL). Both olive rHPLs are relatively stable at 4 °C, and enzyme activity can be preserved (about...

journal_title：Applied biochemistry and biotechnology

authors： Jacopini S,Vincenti S,Mariani M,Brunini-Bronzini de Caraffa V,Gambotti C,Desjobert JM,Muselli A,Costa J,Tomi F,Berti L,Maury J

更新日期：2017-07-01 00:00:00

abstract：:Calotropis procera latex fractions possessing anti-inflammatory property were characterized for their biochemical properties, compared for their efficacy in ameliorating fever in rats and their mechanism of action was elucidated. Aqueous fraction and methanol extract (AqDL and MeDL) were derived from the dried latex (...

journal_title：Applied biochemistry and biotechnology

authors： Kumar VL,Guruprasad B,Fatmi SMA,Chaudhary P,Alencar NMN,Lima-Filho JVM,Ramos MV

更新日期：2017-07-01 00:00:00

abstract：:In the present study, an intracellular melanin, named LIM205, was separated from Lachnum YM205 mycelia and was purified on a Sephadex G-15 column. The molecular weight of LIM205 was determined as 522 Da, and its molecular formula was speculated as C28H14N2O7S. The possible chemical structure of LIM205 was determined a...

journal_title：Applied biochemistry and biotechnology

authors： Zong S,Li L,Li J,Shaikh F,Yang L,Ye M

更新日期：2017-06-01 00:00:00

abstract：:New approaches to veterinary drug screening based on liquid chromatography-mass spectrometry (LC-MS/MS) and time-of-flight mass spectrometry (ToF/MS) are rapid and have high selectivity and sensitivity. In this study, we developed a multiresidue method for screening over 100 veterinary drug residues using ion trap (IT...

journal_title：Applied biochemistry and biotechnology

authors： Kang J,Park SJ,Park HC,Hossain MA,Kim MA,Son SW,Lim CM,Kim TW,Cho BH

更新日期：2017-06-01 00:00:00

abstract：:Aspergillus fumigatus N2 was isolated from decaying wood. This strain produces extracellular xylanases and cellulases. The highest xylanase (91.9 U/mL) and CMCase (5.61 U/mL) activity was produced when 1% barley straw was used as the carbon source. The optimum pH and temperature for xylanase activity were 6.0 and 65 °...

journal_title：Applied biochemistry and biotechnology

authors： Lin C,Shen Z,Qin W

更新日期：2017-06-01 00:00:00

abstract：:A novel β-1,3-glucanase gene, designated Ccglu17A, was cloned from the biological control fungus Chaetomium cupreum Ame. Its 1626-bp open reading frame encoded 541 amino acids. The corresponding amino acid sequence showed highest identity (67 %) with a glycoside hydrolase family 17 β-1,3-glucanase from Chaetomium glob...

journal_title：Applied biochemistry and biotechnology

authors： Jiang C,Song J,Cong H,Zhang J,Yang Q

更新日期：2017-05-01 00:00:00

abstract：:Biodegradation is one of the important methods for the treatment of industrial wastewater containing aniline. In this paper, a degrading bacterium named MC-01, which could survive in high concentration aniline wastewater, was screened from industrial wastewater containing aniline and sludge. MC-01 was preliminarily id...

journal_title：Applied biochemistry and biotechnology

authors： Yang L,Ying C,Fang N,Zhong Y,Zhao-Xiang Z,Yun S

更新日期：2017-05-01 00:00:00

abstract：:The objective of this study was to evaluate the capability of a lipopolysaccharide, produced by Trichosporon mycotoxinivorans CLA2 using residue of biodiesel processing, to flocculate two different solids in suspension. In addition, the emulsifying activity and the stability of this lipopolysaccharide in response to p...

journal_title：Applied biochemistry and biotechnology

authors： Domingues VS,Monteiro AS,Ferreira GF,Santos VL

更新日期：2017-05-01 00:00:00

abstract：:Solid-state fermentation using the microfungus Penicillium brevicompactum for the production of mycophenolic acid is reported in this paper. Of the initial substrates tested (whole wheat, cracked wheat, long grain Basmati rice, and short grain Parmal rice), Parmal rice proved to be the best. Under initial conditions, ...

journal_title：Applied biochemistry and biotechnology

authors： Patel G,Patil MD,Soni S,Chisti Y,Banerjee UC

更新日期：2017-05-01 00:00:00

abstract：:A combined process, de-algination followed by enzymatic saccharification, was designed to produce alginate and glucose from Saccharina japonica consecutively. The process conditions of de-algination were optimized separately for each stage of acidification and alkaline extraction. Collectively, the de-algination yield...

journal_title：Applied biochemistry and biotechnology

authors： Ryu HJ,Oh KK

更新日期：2017-05-01 00:00:00

abstract：:cDNA coding a prolyl aminopeptidase (PAP) was cloned from Aspergillus oryzae and over expressed in Bacillus subtilis with a 6×His tag in N-terminus. The recombinant prolyl aminopeptidase was secreted to extracellular by adding 2 mM CaCl2 and 5% D-sorbitol in TB medium; the enzyme activity in fermented supernatant incr...

journal_title：Applied biochemistry and biotechnology

authors： Wang KD,Wang KH,Zhou ND,Tian YP

更新日期：2017-04-01 00:00:00

abstract：:Streptomyces badius DB-1 produces α-amylase extracellularly, and its production was enhanced 5.1-fold (from 9.47 ± 0.51 to 48.23 ± 1.45 U mL-1) due to optimization by one-variable-at-a-time and statistical approaches. Soluble starch emerged as the most influential factor that strongly affected enzyme production. The p...

journal_title：Applied biochemistry and biotechnology

authors： Shivlata L,Satyanarayana T

更新日期：2017-04-01 00:00:00

abstract：:Prostate cancer is one of the most common malignancies among men worldwide. The main aim of the present work was to clarify the advantages of a nanoformulation of ayurvedic herbal plants. Specifically, we assessed the improved anticancer activity of Leucas aspera nanoparticles compared with methanolic crude extract in...

journal_title：Applied biochemistry and biotechnology

authors： Mohan A,Nair SV,Lakshmanan VK

更新日期：2017-04-01 00:00:00

abstract：:In this study, the gene encoding an α-amylase from a psychrophilic Arthrobacter agilis PAMC 27388 strain was cloned into a pET-28a(+) vector and heterologously expressed in Escherichia coli BL21(DE3). The recombinant α-amylase with a molecular mass of about 80 kDa was purified by using Ni2+-NTA affinity chromatography...

journal_title：Applied biochemistry and biotechnology

authors： Kim SM,Park H,Choi JI

更新日期：2017-03-01 00:00:00

abstract：:Lignocellulosic feedstocks corn stover, prairie cord grass, and switchgrass were subjected to ammonia fiber expansion (AFEX™) pretreatment and densified using extrusion pelleting and ComPAKco densification technique. The effects of AFEX™ pretreatment and densification were studied on the fast pyrolysis product yields....

journal_title：Applied biochemistry and biotechnology

authors： Sundaram V,Muthukumarappan K,Gent S

更新日期：2017-03-01 00:00:00